This is the Linux app named SSC whose latest release can be downloaded as SSC0.1.tar.gz. It can be run online in the free hosting provider OnWorks for workstations.
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SSC
DESCRIPTION:
SSC is a tool for predicting sgRNA efficiency from spacer sequences. It supports the applications of optimizing sgRNA libraries in CRISPR/Cas9 knockout or CRISPR/dCas9 inhibition/activation screens.Features
- Extracting candidate spacer sequences from .fasta file
- Predict sgRNA efficiency from the spacer sequences, for library design in CRISPR/Cas9 knockout and CRISPR/dCas9 inhibition or activation.
- provide matrices that supports: 19-20 bps spacers in CRISPR/Cas9 knockout, optimized for human and mouse genome.
- provide matrices that supports: 19-21 bps spacers in CRISPR/dCas9, optimized for human genome.
This is an application that can also be fetched from https://sourceforge.net/projects/spacerscoringcrispr/. It has been hosted in OnWorks in order to be run online in an easiest way from one of our free Operative Systems.