This is the command glam2 that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
glam2 - Gapped Local Alignment of Motifs
SYNOPSIS
glam2 [options] alphabet my_seqs.fa
An alphabet other than p or n is interpreted as the name of an alphabet file.
DESCRIPTION
GLAM2 is a software package for finding motifs in sequences, typically amino-acid or
nucleotide sequences. A motif is a re-occurring sequence pattern: typical examples are the
TATA box and the CAAX prenylation motif. The main innovation of GLAM2 is that it allows
insertions and deletions in motifs.
OPTIONS (DEFAULT SETTINGS)
-h
Show all options and their default settings.
-o
Output file (stdout).
-r
Number of alignment runs (10).
-n
End each run after this many iterations without improvement (10000).
-2
Examine both strands - forward and reverse complement.
-z
Minimum number of sequences in the alignment (2).
-a
Minimum number of aligned columns (2).
-b
Maximum number of aligned columns (50).
-w
Initial number of aligned columns (20).
-d
Dirichlet mixture file.
-D
Deletion pseudocount (0.1).
-E
No-deletion pseudocount (2.0).
-I
Insertion pseudocount (0.02).
-J
No-insertion pseudocount (1.0).
-q
Weight for generic versus sequence-set-specific residue abundances (1e+99).
-t
Initial temperature (1.2).
-c
Cooling factor per n iterations (1.44).
-u
Temperature lower bound (0.1).
-p
Print progress information at each iteration.
-m
Column-sampling moves per site-sampling move (1.0).
-x
Site sampling algorithm: 0=FAST 1=SLOW 2=FFT (0).
-s
Seed for pseudo-random numbers (1).
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