This is the Linux app named Gemi whose latest release can be downloaded as Gemiv1.5.0.zip. It can be run online in the free hosting provider OnWorks for workstations.
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SCREENSHOTS
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Gemi
DESCRIPTION
Please, do not hesitate to contact Dr. Haitham Sobhy if you need help.
Gemi, an automated, fast, and easy-to-use bioinformatics tool with a user-friendly interface to design primers and probes for polymerase chain reaction (PCR). Gemi accepts multiple aligned and long DNA and RNA sequences with degenerate nucleotide (non-A/C/G/T bases). Gemi can be used for quantitative, real-time and conventional PCR (qPCR, rt-PCR, etc.), and Sanger sequencing. Gemi can parse large dataset of sequences efficiently.
Article:
Gemi: PCR primers prediction from multiple alignments
Comparative and functional genomics 2012;2012:783138
DOI: https://doi.org/10.1155/2012/783138
PMID: https://www.ncbi.nlm.nih.gov/pubmed/23316117
PMCID: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3535827/
Audience
Agriculture, Education, Government, Science/Research
User interface
.NET/Mono, Win32 (MS Windows)
Programming Language
C#
Categories
This is an application that can also be fetched from https://sourceforge.net/projects/gemi/. It has been hosted in OnWorks in order to be run online in an easiest way from one of our free Operative Systems.
