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diffseq - Compare and report features of two similar sequences


diffseq -asequence sequence -bsequence sequence -wordsize integer
[-globaldifferences boolean] -outfile report -aoutfeat featout -boutfeat featout

diffseq -help


diffseq is a command line program from EMBOSS (“the European Molecular Biology Open
Software Suite”). It is part of the "Alignment:Differences" command group(s).


Input section
-asequence sequence

-bsequence sequence

Required section
-wordsize integer
The similar regions between the two sequences are found by creating a hash table of
'wordsize'd subsequences. 10 is a reasonable default. Making this value larger (20?)
may speed up the program slightly, but will mean that any two differences within
'wordsize' of each other will be grouped as a single region of difference. This value
may be made smaller (4?) to improve the resolution of nearby differences, but the
program will go much slower. Default value: 10

Additional section
-globaldifferences boolean
Normally this program will find regions of identity that are the length of the
specified word-size or greater and will then report the regions of difference between
these matching regions. This works well and is what most people want if they are
working with long overlapping nucleic acid sequences. You are usually not interested
in the non-overlapping ends of these sequences. If you have protein sequences or short
RNA sequences however, you will be interested in differences at the very ends . It
this option is set to be true then the differences at the ends will also be reported.
Default value: N

Output section
-outfile report

-aoutfeat featout
File for output of first sequence's features Default value: $(asequence.name).diffgff

-boutfeat featout
File for output of second sequence's features Default value: $(bsequence.name).diffgff

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