This is the command digeste that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
digest - Reports on protein proteolytic enzyme or reagent cleavage sites
digest -seqall seqall -mwdata datafile -menu list -mono boolean -unfavoured boolean
-ragging boolean -termini list -overlap boolean -allpartials boolean
digest is a command line program from EMBOSS (“the European Molecular Biology Open
Software Suite”). It is part of the "Protein:Motifs" command group(s).
Molecular weight data for amino acids Default value: Emolwt.dat
Default value: 1
Default value: N
Trypsin will not normally cut after 'KR' if they are followed by any of 'KRIFLP'.
Lys-C will not normally cut after 'K' if it is followed by 'P'. Arg-C will not
normally cut after 'R' if it is followed by 'P'. V8-bicarb will not normally cut after
'E' if it is followed by any of 'KREP'. V8-phosph will not normally cut after 'DE' if
they are followed by 'P'. Chymotrypsin will not normally cut after 'FYWLM' if they are
followed by 'P'. Specifying unfavoured shows these unfavoured cuts as well as the
Allows semi-specific and non-specific digestion. This option is particularly useful
for generating lists of peptide sequences for protein identification using
Default value: 1
Used for partial digestion. Shows all cuts from favoured cut sites plus 1..3, 2..4,
3..5 etc but not (e.g.) 2..5. Overlaps are therefore fragments with exactly one
potential cut site within it.
As for overlap but fragments containing more than one potential cut site are included.
Use digeste online using onworks.net services