This is the command digeste that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
digest - Reports on protein proteolytic enzyme or reagent cleavage sites
SYNOPSIS
digest -seqall seqall -mwdata datafile -menu list -mono boolean -unfavoured boolean
-ragging boolean -termini list -overlap boolean -allpartials boolean
-outfile report
digest -help
DESCRIPTION
digest is a command line program from EMBOSS (“the European Molecular Biology Open
Software Suite”). It is part of the "Protein:Motifs" command group(s).
OPTIONS
Input section
-seqall seqall
-mwdata datafile
Molecular weight data for amino acids Default value: Emolwt.dat
Required section
-menu list
Default value: 1
-mono boolean
Default value: N
Advanced section
-unfavoured boolean
Trypsin will not normally cut after 'KR' if they are followed by any of 'KRIFLP'.
Lys-C will not normally cut after 'K' if it is followed by 'P'. Arg-C will not
normally cut after 'R' if it is followed by 'P'. V8-bicarb will not normally cut after
'E' if it is followed by any of 'KREP'. V8-phosph will not normally cut after 'DE' if
they are followed by 'P'. Chymotrypsin will not normally cut after 'FYWLM' if they are
followed by 'P'. Specifying unfavoured shows these unfavoured cuts as well as the
favoured ones.
-ragging boolean
Allows semi-specific and non-specific digestion. This option is particularly useful
for generating lists of peptide sequences for protein identification using
mass-spectrometry.
-termini list
Default value: 1
Output section
-overlap boolean
Used for partial digestion. Shows all cuts from favoured cut sites plus 1..3, 2..4,
3..5 etc but not (e.g.) 2..5. Overlaps are therefore fragments with exactly one
potential cut site within it.
-allpartials boolean
As for overlap but fragments containing more than one potential cut site are included.
-outfile report
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