This is the command fastaq-to_perfect_reads that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
fastaq_to_perfect_reads - Make perfect paired reads from reference
DESCRIPTION
usage: fastaq_to_perfect_reads [options] <infile> <outfile> <mean insert size> <insert std
deviation> <mean coverage> <read length>
Makes perfect paired end fastq reads from a sequence file, with insert sizes sampled from
a normal distribution. Read orientation is innies. Output is an interleaved FASTQ file.
positional arguments:
infile Name of input file
outfile
Name of output file
mean insert size
Mean insert size of read pairs
insert std deviation
Standard devation of insert size
mean coverage
Mean coverage of the reads
read length
Length of each read
optional arguments:
-h, --help
show this help message and exit
--fragments FILENAME
Write FASTA sequences of fragments (i.e. read pairs plus sequences in between them)
to the given filename
--no_n Don't allow any N or n characters in the reads
--seed INT
Seed for random number generator. Default is to use python's default
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