This is the command macs2_pileup that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
macs2_pileup - Model-based Analysis for ChIP-Sequencing
DESCRIPTION
usage: macs2 pileup [-h] -i IFILE [IFILE ...] -o OUTPUTFILE [--outdir OUTDIR]
[-f {AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE}]
[-B] [--extsize EXTSIZE] [--verbose VERBOSE]
optional arguments:
-h, --help
show this help message and exit
-i IFILE [IFILE ...], --ifile IFILE [IFILE ...]
ChIP-seq alignment file. If multiple files are given as '-t A B C', then they will
all be read and combined. Note that pair-end data is not supposed to work with this
command. REQUIRED.
-o OUTPUTFILE, --ofile OUTPUTFILE
Output bedGraph file name. If not specified, will write to standard output.
REQUIRED.
--outdir OUTDIR
If specified all output files will be written to that directory. Default: the
current working directory
-f {AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE}, --format
{AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE}
Format of tag file, "AUTO", "BED" or "ELAND" or "ELANDMULTI" or "ELANDEXPORT" or
"SAM" or "BAM" or "BOWTIE". The default AUTO option will let 'macs2 pileup' decide
which format the file is. Please check the definition in README file if you choose
ELAND/ELANDMULTI/ELANDEXPORT/SAM/BAM/BOWTIE. DEFAULT: "AUTO"
-B, --both-direction
By default, any read will be extended towards downstream direction by extension
size. So it's [0,size-1] (1-based index system) for plus strand read and
[-size+1,0] for minus strand read where position 0 is 5' end of the aligned read.
Default behavior can simulate MACS2 way of piling up ChIP sample reads where
extension size is set as fragment size/d. If this option is set as on, aligned
reads will be extended in both upstream and downstream directions by extension
size. It means [-size,size] where 0 is the 5' end of a aligned read. It can
partially simulate MACS2 way of piling up control reads. However MACS2 local bias
is calculated by maximizing the expected pileup over a ChIP fragment size/d
estimated from 10kb, 1kb, d and whole genome background. DEFAULT: False
--extsize EXTSIZE
The extension size in bps. Each alignment read will become a EXTSIZE of fragment,
then be piled up. Check description for -B for detail. It's twice the `shiftsize`
in old MACSv1 language. DEFAULT: 200
--verbose VERBOSE
Set verbose level. 0: only show critical message, 1: show additional warning
message, 2: show process information, 3: show debug messages. If you want to know
where are the duplicate reads, use 3. DEFAULT:2
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