This is the command toAfg that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
toAfg - Print reads stored in a file (pls|fasta|fastq) as an afg.
toAfg input.filetype output.filetype [-minSubreadLength l] [-regionTable regions_file]
[-noSplitSubreads] [-useccsdenovo] [-uniformQV QV]
Use toAfg online using onworks.net services