This is the command genome-music-path-scanp that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
genome music path-scan - Find signifcantly mutated pathways in a cohort given a list of
This document describes genome music path-scan version 0.04 (2016-01-01 at 23:10:18)
genome music path-scan --gene-covg-dir=? --bam-list=? --pathway-file=? --maf-file=?
--output-file=? [--bmr=?] [--genes-to-ignore=?] [--min-mut-genes-per-path=?]
... music path-scan \
--bam-list input_dir/bam_file_list \
--gene-covg-dir output_dir/gene_covgs/ \
--maf-file input_dir/myMAF.tsv \
--output-file output_dir/sm_pathways \
--pathway-file input_dir/pathway_dbs/KEGG.txt \
Directory containing per-gene coverage files (Created using music bmr calc-covg)
Tab delimited list of BAM files [sample_name, normal_bam, tumor_bam] (See Description)
Tab-delimited file of pathway information (See Description)
List of mutations using TCGA MAF specifications v2.3
Output file that will list the significant pathways and their p-values
Background mutation rate in the targeted regions
Default value '1e-06' if not specified
Comma-delimited list of genes whose mutations should be ignored
Pathways with fewer mutated genes than this, will be ignored
Default value '1' if not specified
Skip non-coding mutations from the provided MAF file
Default value 'true' if not specified
Make skip-non-coding 'false'
Skip silent mutations from the provided MAF file
Default value 'true' if not specified
Make skip-silent 'false'
Only the following four columns in the MAF are used. All other columns may be left blank.
Col 1: Hugo_Symbol (Need not be HUGO, but must match gene names used in the pathway file)
Col 2: Entrez_Gene_Id (Matching Entrez ID trump gene name matches between pathway file and MAF)
Col 9: Variant_Classification
Col 16: Tumor_Sample_Barcode (Must match the name in sample-list, or contain it as a substring)
The Entrez_Gene_Id can also be left blank (or set to 0), but it is highly recommended, in
case genes are named differently in the pathway file and the MAF file.
This is a tab-delimited file prepared from a pathway database (such as KEGG), with the
columns: [path_id, path_name, class, gene_line, diseases, drugs, description] The
latter three columns are optional (but are available on KEGG). The gene_line contains
the "entrez_id:gene_name" of all genes involved in this pathway, each separated by a
For example, a line in the pathway-file would look like:
hsa00061 Fatty acid biosynthesis Lipid Metabolism 31:ACACA|32:ACACB|27349:MCAT|2194:FASN|54995:OXSM|55301:OLAH
Ensure that the gene names and entrez IDs used match those used in the MAF
file. Entrez IDs are not mandatory (use a 0 if Entrez ID unknown). But if a
gene name in the MAF does not match any gene name in this file, the entrez IDs
are used to find a match (unless it's a 0).
This is usually the gene_covgs subdirectory created when you run "music bmr calc-
covg". It should contain files for each sample that report per-gene covered base
Provide a file containing sample names and normal/tumor BAM locations for each. Use
the tab- delimited format [sample_name normal_bam tumor_bam] per line. This tool only
needs sample_name, so all other columns can be skipped. The sample_name must be the
same as the tumor sample names used in the MAF file (16th column, with the header
The overall background mutation rate. This can be calculated using "music bmr calc-
A comma-delimited list of genes to ignore from the MAF file. This is useful when there
are recurrently mutated genes like TP53 which might mask the significance of other
Michael Wendl, Ph.D.
This module uses reformatted copies of data from the Kyoto Encyclopedia of Genes and
Genomes (KEGG) database:
* KEGG - http://www.genome.jp/kegg/
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