This is the command macs2_refinepeak that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
macs2_refinepeak - Model-based Analysis for ChIP-Sequencing
DESCRIPTION
usage: macs2 refinepeak [-h] -b BEDFILE -i IFILE [IFILE ...]
[-f {AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE}]
[-c CUTOFF] [-w WINDOWSIZE] [--verbose VERBOSE] [--outdir OUTDIR] (-o OFILE |
--o-prefix OPREFIX)
optional arguments:
-h, --help
show this help message and exit
-b BEDFILE
Candidate peak file in BED format. REQUIRED.
-i IFILE [IFILE ...], --ifile IFILE [IFILE ...]
ChIP-seq alignment file. If multiple files are given as '-t A B C', then they will
all be read and combined. Note that pair-end data is not supposed to work with this
command. REQUIRED.
-f {AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE}, --format
{AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE}
Format of tag file, "AUTO", "BED" or "ELAND" or "ELANDMULTI" or "ELANDEXPORT" or
"SAM" or "BAM" or "BOWTIE". The default AUTO option will let 'macs2 refinepeak'
decide which format the file is. Please check the definition in README file if you
choose ELAND/ELANDMULTI/ELANDEXPORT/SAM/BAM/BOWTIE. DEFAULT: "AUTO"
-c CUTOFF, --cutoff CUTOFF
Cutoff DEFAULT: 5
-w WINDOWSIZE, --window-size WINDOWSIZE
Scan window size on both side of the summit (default: 100bp)
--verbose VERBOSE
Set verbose level. 0: only show critical message, 1: show additional warning
message, 2: show process information, 3: show debug messages. If you want to know
where are the duplicate reads, use 3. DEFAULT:2
--outdir OUTDIR
If specified all output files will be written to that directory. Default: the
current working directory
-o OFILE, --ofile OFILE
Output file name. Mutually exclusive with --o-prefix.
--o-prefix OPREFIX
Output file prefix. Mutually exclusive with -o/--ofile.
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