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makehmmerdb - build a HMMER binary database file from a sequence file


makehmmerdb [options] <seqfile> <binaryfile>


makehmmerdb is used to create a binary file from a DNA sequence file. This binary file may
be used as a target database for the DNA search tool nhmmer.Usingdefaultsettingsin nhmmer,
this yields a roughly 10-fold acceleration with small loss of sensitivity on benchmarks.
(This method has been extensively tested, but should still be treated as somewhat


-h Help; print a brief reminder of command line usage and all available options.


--informat <s>
Assert that the sequence database file is in format <s>. Accepted formats include
fasta, embl, genbank, ddbj, uniprot, stockholm, pfam, a2m, and afa. The default is
to autodetect the format of the file.

--bin_length <n>
Bin length. The binary file depends on a data structure called the FM index, which
organizes a permuted copy of the sequence in bins of length <n>. Longer bin length
will lead to smaller files (because data is captured about each bin) and possibly
slower query time. The default is 256. Much more than 512 may lead to notable
reduction in speed.

--sa_freq <n>
Suffix array sample rate. The FM index structure also samples from the underlying
suffix array for the sequence database. More frequent sampling (smaller value for
<n>) will yield larger file size and faster search (until file size becomes large
enough to cause I/O to be a bottleneck). The default value is 8. Must be a power of

--block_size <n>
The input sequence is broken into blocks of size <n> million letters. An FM index
is built for each block, rather than building an FM index for the entire sequence
database. Default is 50. Larger blocks do not seem to yield substantial speed

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