This is the command perlprimer that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
perlprimer - graphically specify amplicon of DNA or mRNA sequences and design primers
SYNOPSIS
perlprimer
DESCRIPTION
PerlPrimer calculates primer melting temperature using J. SantaLucia's extensive nearest-
neighbour thermodynamic parameters. To adjust for the salt conditions of the PCR,
PerlPrimer uses the empirical formula derived by von Ahsen, et al. (2001) and allows the
user to specify the concentration of Mg2+, dNTPs and primers, or use standard PCR
conditions. The result is a highly accurate prediction of primer melting temperature,
giving rise to a maximum yeild of product when amplified. PerlPrimer is written in Perl
and requires Perl/Tk. In addition, for QPCR functionality PerlPrimer requires the open-
source Spidey executable from NCBI. The program is designed to be cross-platform
compatible and has been developed and tested on both Microsoft Windows and GNU/Linux-based
operating systems. Users have also reported success using the program under Mac OS X.
Use perlprimer online using onworks.net services
