This is the command tbl2asn that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator
PROGRAM:
NAME
tbl2asn - prepare a GenBank submission using an ASCII feature table
SYNOPSIS
tbl2asn [-] [-A str] [-C str] [-D filename] [-E] [-F str] [-G str] [-H str] [-J] [-K] [-L]
[-M str] [-N n] [-O] [-P] [-Q str] [-R] [-S] [-T] [-U] [-V str] [-W] [-X str]
[-Y filename] [-Z filename] [-a str] [-b] [-c str] [-f filename] [-g] [-h] [-i filename]
[-j str] [-k str] [-n str] [-o filename] [-p str] [-q] [-r str] [-s] [-t filename] [-u]
[-v] [-w filename] [-x str] [-y str] [-z]
DESCRIPTION
tbl2asn reads a template along with sequence and table files, and outputs ASN.1 for
submission to GenBank. Thus, the submitter does not need to read each set of table and
sequence files into Sequin. Furthermore, the template file can contain the organism and
submitter information common to all records, obviating the need to input these data for
each sequence/table pair.
OPTIONS
A summary of options is included below.
- Print usage message
-a str Accession
-C str Genome Center tag
-D filename
Descriptors file
-E Recurse
-F Feature ID links (o by Overlap, p by Product)
-G str Alignment Gap Flags (comma separated fields, e.g., p,-,-,-,?,. ) n Nucleotide or p
Protein, Begin, Middle, End Gap Characters, Missing Characters, Match Characters
Alignment middle Gap characters
-H str Hold until publication:
y For one year
mm/dd/yyyy
Until the specified date
-J Delayed genomic product set
-K Safe Bioseq-set
-L Force Local protein_id/transcript_id
-M str Master genome flags:
n Normal
b Big sequence
p Power option
t TSA
-N n Project version number
-O Allow run-on ORFs
-P Remote publication lookup
-Q mRNA title policy
s Special mRNA titles
r RefSeq mRNA titles
-R Remote sequence record fetching from ID
-S Smart feature annotation
-T Remote Taxonomy lookup
-U Remove Unnecessary gene xref
-V str Verification (combine any of the following letters)
v Validate with Normal Stringency
r Validate without Country Check
c BarCode validation
b Generate GenBank Flatfile
g Generate Gene Report
t Validate with TSA check
-W Log progress
-X str Extra flags (combine any of the following letters)
A Automatic definition line generation
C Apply Comments in .cmt files to all sequences
E Treat like Eukarypota in the discrepancy report
-Y filename
Read a comment string from filename
-Z filename
Write a discrepancy report to filename
-a str File type:
a Any (default)
r20u Runs of 20+ Ns are gaps, 100 Ns are unknown length
r20k Runs of 20+ Ns are gaps, 100 Ns are known length
r10u Runs of 10+ Ns are gaps, 100 Ns are unknown length
r10k Runs of 10+ Ns are gaps, 100 Ns are known length
s FASTA Set (s Batch, s1 Pop, s2 Phy, s3 Mut, s4 Eco, s9 Small-genome)
d FASTA Delta
di FASTA Delta with Implicit Gaps
l FASTA+Gap Alignment (l Batch, l1 Pop, l2 Phy, l3 Mut, l4 Eco, l9 Small-
genome)
z FASTA with Gap Lines
e PHRAP/ACE -
b ASN.1 (in conjunction with M
)
-b Generate GenBank file (deprecated in favor of -V b)
-c str Cleanup (combine any of the following letters)
d Correct Collection Dates (assume month first)
D Correct Collection Dates (assume day first)
b Append note to coding regions that overlap other coding regions with similar
product names and do not contain 'ABC'
x Extend partial ends of features by one or two nucleotides to abut gaps or
sequence ends
s Add exception to short introns
f Fix product names
-f filename
Single table file
-g Input is a genomic product set
-h Convert general ID to note
-i filename
Single input file
-j str Source qualifiers
-k str CDS flags (combine any of the following letters)
c Annotate Longest ORF
r Allow Runon ORFs
m Allow Alternative Starts
k Set Conflict on Mismatch
-n str Organism name
-o filename
Single output file
-p str Path to files
-q Set sequence ID from input file name
-r str Path for results
-s Read FASTAs as Set
-t filename
Read template from filename
-u Convert GenProdSet to NucProtSet
-v Validate (deprecated in favor of -V v)
-w filename
Single structured comment file
-x str Suffix (default = .fsa)
-y str
-z Clean up log file Comment
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