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mhap - Online in the Cloud

Run mhap in OnWorks free hosting provider over Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator

This is the command mhap that can be run in the OnWorks free hosting provider using one of our multiple free online workstations such as Ubuntu Online, Fedora Online, Windows online emulator or MAC OS online emulator

PROGRAM:

NAME


mhap - probabilistic sequence overlapping

DESCRIPTION


Please set the -s or the -p options. See options below: MHAP: MinHash Alignment Protocol.
A tool for finding overlaps of long-read sequences (such as PacBio or Nanopore) in
bioinformatics.

Version: 1.6, Build time: 09/12/2015 11:46 PM Usage 1 (direct execution): java
-server -Xmx<memory> -jar <MHAP jar> -s<fasta/dat from/self file> [-q<fasta/dat to
file>] [-f<kmer filter list, must be sorted>] Usage 2 (generate precomputed
binaries): java -server -Xmx<memory> -jar <MHAP jar> -p<directory of fasta files>
-q <output directory> [-f<kmer filter list, must be sorted>]

--alignment, default = false
Experimental option.

--alignment-offset, default = -0.535
The offset to account for the variance in the alignment match score.

--alignment-score, default = 1.0E-6
The cutoff score for alignment matches.

--filter-threshold, default = 1.0E-5
[double], the cutoff at which the k-mer in the k-mer filter file is considered
repetitive. This value for a specific k-mer is specified in the second column in
the filter file. If no filter file is provided, this option is ignored.

--help, default = false
Displays the help menu.

--max-shift, default = 0.2
[double], region size to the left and right of the estimated overlap, as derived
from the median shift and sequence length, where a k-mer matches are still
considered valid. Second stage filter only.

--min-store-length, default = 0
[int], The minimum length of the read that is stored in the box. Used to filter out
short reads from FASTA file.

--nanopore-fast, default = false
Set all the parameters for the Nanopore fast settings. This is the current best
guidance, and could change at any time without warning.

--no-self, default = false
Do not compute the overlaps between sequences inside a box. Should be used when the
to and from sequences are coming from different files.

--num-hashes, default = 512
[int], number of min-mers to be used in MinHashing.

--num-min-matches, default = 3
[int], minimum # min-mer that must be shared before computing second stage filter.
Any sequences below that value are considered non-overlapping.

--num-threads, default = 12
[int], number of threads to use for computation. Typically set to 2 x #cores.

--pacbio-fast, default = false
Set all the parameters for the PacBio fast setting. This is the current best
guidance, and could change at any time without warning.

--pacbio-sensitive, default = false
Set all the parameters for the PacBio sensitive settings. This is the current best
guidance, and could change at any time without warning.

--store-full-id, default = false
Store full IDs as seen in FASTA file, rather than storing just the sequence
position in the file. Some FASTA files have long IDS, slowing output of results.
This options is ignored when using compressed file format.

--threshold, default = 0.04
[double], the threshold similarity score cutoff for the second stage sort-merge
filter. This is based on the average number of k-mers matching in the overlapping
region.

--version, default = false
Displays the version and build time.

--weighted, default = false
Perform weighted MinHashing.

-f, default = ""
k-mer filter file used for filtering out highly repetative k-mers. Must be sorted
in descending order of frequency (second column).

-h, default = false
Displays the help menu.

-k, default = 16
[int], k-mer size used for MinHashing. The k-mer size for second stage filter is
separate, and cannot be modified.

-p, default = ""
Usage 2 only. The directory containing FASTA files that should be converted to
binary format for storage.

-q, default = ""
Usage 1: The FASTA file of reads, or a directory of files, that will be compared to
the set of reads in the box (see -s). Usage 2: The output directory for the binary
formatted dat files.

-s, default = ""
Usage 1 only. The FASTA or binary dat file (see Usage 2) of reads that will be
stored in a box, and that all subsequent reads will be compared to.

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